Liquid Media Preparation

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  • Prepare XmL of 1.5% Glucose (Final Concentration), 1x Vogel's (Final Concentration) liquid media for each media/strain combination to be used and add or substitute additional media components as necessary.
  • Pipette a XmL aliquot of liquid media into an appropriately sized Erlenmeyer Flask (approximately 2.5 to 5 times the volume of the aliquot) and cap with foam plugs.
  • Autoclave media and allow to cool to room temperature and store on bench.

Note: When preparing media, allow it to cool to room temp after autoclaving before use.

Note: Heat sensitive media components (such as Ethanol, MMS, FGS) can be added after autoclaving from a sterile source before the media solidifies.


Example: 50mL Liquid Cultures

  • Prepare one 50mL aliquot of 1.5% Glucose (.75g), 1x Vogel's (1mL 50X Vogel's) liquid media for each media/strain combination to be used and add additional media components as necessary.
  • Pipette liquid media into 125mL Erlenmeyer Flasks in 50mL aliquots and cap with foam plugs.
  • Autoclave media and allow to cool to room temperature and store on bench.



Posted by Michael Seymour 2013