RNAse A (10mg/ml)

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RNase A (10mg/mL) Stock Preparation

  1. Measure out 10mg of RNase A powder into a sterile 1.5mL epi-tube
  2. Add 1mL of PBS (from sterile lab stocks) for a final concentration of 10mg/mL
  3. vortex solution till all powder is dissolved
  4. Carefully pipette resulting solution into 100uL aliquots in new sterile 1.5mL epi-tubes (should make 10)
  5. Clearly label Aliquots on both the top and sides, and store in -200C Freezer until needed

Posted by Michael Seymour 2013