Southern Hybridization Buffer

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Also known as SDS prehybe buffer (7% SDS, 250mM NaPO4, 1% BSA, 1mM EDTA)


For 1L, dissolve the following in 600ml H2O:

SDS 70g
1M NaPO4 pH 6.8 250ml
BSA 10g
0.5M EDTA 2ml
to 1000ml


Recipe for 1M NaPO4 pH 6.8

Monobasic NaH2PO4 140.76g
dibasic Na2HPO4 139.16g

add the dibasic after the monobasic is dissolved