TCA/EtOH Solution

From Lewis Lab Wiki
Jump to: navigation, search

TCA/ EtOH SOLUTION

  1. make 4.5 M TCA by dissolving 417 g NaTCA salt (Aldrich #19,078-0) in H2O until 500 ml are reached (density is 1.43 g/ml, i.e. wt of 50 ml should be 71.5 g)
  2. add 500 ml EtOH, store at 4° C (precipitate will appear upon mixing and settles; don't worry, but don't mix it with the clear solution when using)